MaxLight™ 405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Shank is a novel family of postsynaptic density proteins (PSD) that bind to PSD-95-associated protein GKAP.
Its putative function is to act as a scaffold protein in the PSD to cross-link NMDA receptors/PSD-95 complexes and couple them to regulators of the actin cytoskeleton.
Shank1a is a splice variant of Shank1.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:100-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity:Recognizes SHANK1a.
Isotype:IgG
Calc Applications Abbrev:IHC WB
Immunogen:SGPIYPGLFDIRSS, from the C-terminus of Shank1a.