MaxLight™ 405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Myc gene encodes a transcription factor that is believed to regulate expression of 15% of all genes through binding of Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs).
This means that in addition to its role as a classical transcription factor, Myc also functions to regulate global chromatin structure.
Applications:Suitable for use in FLISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:FLISA (coating): 1:100-1:500FLISA (primary): 1:1000-1:30,000Western Blot (colorimetric): 1:1000-1:10,000Western Blot (chemiluminescent): 1:1000-1:30,000Immunocytochemistry: 1:100-1:400Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity:Recognizes human C-myc.
Isotype:IgG
Calc Applications Abbrev:FLISA IC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide EQKLISEEDL corresponding to aa410-419 from human myc conjugated to KLH.