MaxLight™ 405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Transforming growth factor-beta (TGFb) family of proteins has potent hematopoietic regulatory properties ranging from effects on the growth and differentiation of primitive stem cells to the differentiated functions of mature cells.
Although most reports have described the inhibitory activities of TGF-beta on hematopoiesis, recent evidence supports the concept that TGF-beta can have both inhibitory and stimulatory actions on these systems.
These differences depend on the differentiation state of the target cell and the other cytokines interacting with the cell.
Furthermore, TGF-beta has direct bidirectional effects on cell surface expression of many cytokine receptors suggesting that it is part of the mechanism of action of TGF-beta.
Applications:Suitable for use in FLISA, Western Blot, Immunohistochemistry, Immunocytochemistry and Neutralization.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: using immersion fixed paraffin-embedded sections of human prostate cancer tissueImmunocytochemistry: using immersion fixed human peripheral blood mononuclear cells (PBMCs) Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography. Endotoxin: <0.1EU/ug (LAL)
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes human LAP (TGF-beta 1). Specific for the LAP derived from the TGF-beta 1 precursor but does not react with LAP from the TGF-beta 2 precursor.
Isotype：IgG
Calc Applications Abbrev：FLISA IC IHC N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to Leu30-Ser390 from human LAP (TGF-beta 1), expressed in CHO cells and S. frugiperda insect ovarian cell line Sf 21.
