Myc gene encodes a transcription factor that is believed to regulate expression of 15% of all genes through binding of Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs).
This means that in addition to its role as a classical transcription factor, Myc also functions to regulate global chromatin structure.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA (coating): 1:100-1:500ELISA (primary): 1:1000-1:30,000Western Blot (colorimetric): 1:1000-1:10,000Western Blot (chemiluminescent): 1:1000-1:30,000Immunocytochemistry: 1:100-1:400Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity:Recognizes human C-myc.
Isotype:IgG
Calc Applications Abbrev:E IC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide EQKLISEEDL corresponding to aa410-419 from human myc conjugated to KLH.