Myc gene encodes a transcription factor that is believed to regulate expression of 15% of all genes through binding of Enhancer Box sequences (E-boxes) and recruiting histone acetyltransferases (HATs).
This means that in addition to its role as a classical transcription factor, Myc also functions to regulate global chromatin structure.
Applications:Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:ELISA (coating): 1:100-1:500ELISA (primary): 1:1000-1:30,000Western Blot (colorimetric): 1:1000-1:10,000Western Blot (chemiluminescent): 1:1000-1:30,000Immunocytochemistry: 1:100-1:400Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human C-myc.
Isotype：IgG
Calc Applications Abbrev：E IC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide EQKLISEEDL corresponding to aa410-419 from human myc conjugated to KLH.
