Saccharomyces cerevisiae is a rapidly growing budding yeast commonly used in brewing and baking.
S.
cerevisiae has been studied intensively as a eukaryotic cell model as it is easy to culture but maintains the internal complexity of plant and animal cells.
Applications:Suitable for use in ELISA, Western Blot and Dot Blot.
Other applications not tested.
Recommended Dilution:Western Blot: ~5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:goat
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity:Reacts with > 40 proteins by Western Blot of solubilized yeast cells.
Isotype:IgG
Calc Applications Abbrev:DB E WB
Calc Crossreactivity:Ye
Immunogen:Solubilized total yeast cells from Saccharomyces cerevisiae.