A large, cytokine-responsive IkappaB kinase (IKK) complex contains 2 subunits, IKK-alpha and IKK-beta, which are protein kinases whose function is needed for NF-kappaB activation by pro-inflammatory stimuli.
IKK is composed of similar amounts of IKK-alpha, and IKK-beta, which are differentially processed forms of a third subunit, IKK-gamma.
IKK-gamma interacts preferentially with IKK-beta and is required for the activation of the IKK complex.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:Recognizes IKK-gamma.
Isotype:IgG
Calc Applications Abbrev:WB
Immunogen:Residues aa385-399 [RRSPPEEPPDFCCPK] of IKK-gamma.