Protein A is a 40-60kD MSCRAMM (microbial surface components recognizing adhesive matrix molecules) surface protein originally found in the cell wall of the bacteria Staphylococcus aureus.
It is encoded by the spa gene and its regulation is controlled by DNA topology, cellular osmolarity, and a two-component system called ArlS-ArlR.
It has found use in biochemical research because of its ability to bind immunoglobulins.
It binds proteins from many of mammalian species, most notably IgGs.
Applications:Suitable for use in FLISA.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
For long-term storage store at 4°C.
Aliquots are stable at 4°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：chicken
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes one precipitin arc against Staphylococcal Protein A.
