Salmonella is a genus of the family Enterobacteriaceae populated by a variety of Gram negative rod-shaped bacteria, many of which are pathogenic and cause a range of diseases in humans.
Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae).
Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria.
This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A.
Applications:Suitable for use in FLISA, Immunohistochemistry, Immunofluorescence and Western Blot..
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes broad range of Salmonella “O” & “H” strains. Antiserum is unadsorbed for Enterobacteriaceae. Does not crossreact with related Enterobacteriaceae.
Isotype：IgG
Calc Applications Abbrev：FLISA IF IHC WB
Immunogen：Mixture of S. enteriditis, S. typhimurium and S. heildelburg
