Human Chorionic Gonadotropin (HCG) is a glycoprotein, which is secreted in large quantities by normal trophoblasts.
It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy.
HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the a- and b-subunits.
The a-subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).
A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP), and HCG, but negative for EMA and AFP is probably a choriocarcinoma.
Applications:Suitable for use in ELISA.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Recommended Pair:Suitable for use with C5069-62J in a sandwich ELISA.
Affinity Constant:3x10e10L/mole
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:Recognizes hCG intact: 100%. Crossreactivity is <0.01% with LH or FSH.