Cathepsin B is a cysteine protease commonly overexpressed in metastisizing cancer cells.
An intracellular (lysosomal) enzyme in peptidase family C1 (papain family), Cathepsin B will hydrolyze proteins with broad specificity for peptide bonds.
Preferentially cleaves -Arg-Arg bonds in small molecule substrates (thus differing from cathepsin L).
In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA (Indirect): 1:1024Western Blot: 1:4000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human liver Cathepsin B. Does not crossreact with Cathepsin L, Cathespin D or Cathepsin H.
