A large, cytokine-responsive IkappaB kinase (IKK) complex contains 2 subunits, IKK-alpha and IKK-beta, which are protein kinases whose function is needed for NF-kappaB activation by pro-inflammatory stimuli.
IKK is composed of similar amounts of IKK-alpha, and IKK-beta, which are differentially processed forms of a third subunit, IKK-gamma.
IKK-gamma interacts preferentially with IKK-beta and is required for the activation of the IKK complex.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity:Recognizes IKK-gamma.
Isotype:IgG
Calc Applications Abbrev:WB
Immunogen:Residues aa2-13 [NRHLWKSQLCEM] of IKK-gamma.