Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis, and other conditions.
The presence of autoantibodies to histones are frequently found in several rheumatic disorders.
In one study, the predominant responses to histones in SLE sera were to H1, H2b, and H3.
Marked elevations of binding occurred to H1 and H2b in 33% of patients, while 25% showed higher binding to H3.
The same study showed the highest anti-histone reactivity to be in patients with rheumatoid arthritis with vasculitis, while the highest reactivity in SLE sera was in those patients with a history of photosensitivity.
In diploid eukaryotic cells, the chromatin fibers are about 20nM in diameter.
They consist of two major components in equal amounts, DNA and basic proteins called histones.
The histones are a group of water and dilute acid soluble basic proteins found associated with DNA in chromosomes.
They are characterized by relatively high levels of lysine and arginine.
Although histones are classified into a limited number of types of fractions with each particular fraction having a fundamentally distinct amino acid composition and sequence, numerous subfractions are observed due to the acetylation, methylation, and phosphorylation of various amino acid residues.
Microheterogeneity or alteration of structure is dynamic such that the histones of a single cell type are found to vary during development.
They are believed to play a role in gene activity and cellular metabolism.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：sheep
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes bovine Histone H2b: 100%. No reaction with Histone-H-1, Histone-H-3 or Histone-H-4. Very low crossreactivity with Histone-H-core.
Isotype：IgG
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Bo
Immunogen：Histone 2B, calf whole protein complexed to ribosomal RNA.
