Human nitric oxide synthases are a family of enzymes responsible for the synthesis of nitric oxide from L-arginine and molecular oxygen.
There are at least three nitric oxide synthases; NOS I, also known as neuronal NOS or nNOS, NOS II, which is referred to as inducible NOS or iNOS and NOS III, also known as endothelial NOS or eNOS.
nNOS plays a role in the upregulation of microglial phospho-p38 MAPK.
Such p38 MAPK activation in microglia is consistent with a potential role in the development of morphine antinociceptive tolerance.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes nNOS.
Isotype：IgG
Calc Applications Abbrev：WB
Immunogen：Residues aa19-31 [PRKGPPLPNGDTEC] of NOS.
