Nestin is an intermediate filament protein that is expressed predominantly in stem cells of the central nervous system in the neural tube.
Upon differentiation, Nestin is replaced by cell type-specific intermediate filaments, (e.g., neurofilaments and glial fibrillary acidic protein (GFAP)).
Nestin expression is re-induced after CNS injury and during regeneration of injured muscle tissue.
Applications:Suitable for use in Flow Cytometry (Not Tested), Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Flow Cytometry (Not Tested): 1:200-1:500Western Blot: 1:500-1:1000Immunohistochemistry: 1:100-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes human Nestin. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Residues aa254-270 [RATEKFQLAVEALEQEK] of 177kD human Nestin protein.
