The receptors for the endothelins are found on various tissues including lung and intestine.
ETA receptor has been reported in a variety of tissues including placenta, uterus, testis, heart, adrenal gland, lung, kidney, ovary, skin, liver, bladder, large blood vessels, cerebellum, neurons, and meningioma tumor cells.
A splice variant has been identified in melanoma (Zhang et al., 1998).
ESTs have been isolated from libraries from the above-mentioned tissues.
Applications:Suitable for Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Suitable for paraffin embedded tissue
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity：Recognizes rat Endothelin A Receptor at ~39kD. Tested using endothelin A receptor transfected COS cells for the expression of the 39kD segment of the cytoplasmic region of the receptor. Species Crossreactivity: human, mouse and sheep.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt Sh
Immunogen：Synthetic peptide corresponding to aa410-422, QEQNHNTERSSHK of rat Endothelin A Receptor (C-Terminal-KLH). MW: 39kD.
