Salmonella is a genus of the family Enterobacteriaceae populated by a variety of Gram negative rod-shaped bacteria, many of which are pathogenic and cause a range of diseases in humans.
Salmonellae possess 3 major surface antigens; the H or flagellar antigen (phase 1 and 2), the O or somatic antigen (part of the LPS moiety) and the Vi or capsular antigen (referred to as K in other Enterobacteriaceae).
Salmonellae also possess the LPS endotoxin characteristic of Gram negative bacteria.
This LPS is composed of an O polysaccharide (O antigen) an R core and the endotoxic inner Lipid A.
Applications:Suitable for use in ELISA, Immunohistochemistry and Western Blot..
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
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Size:100ul
Host:rabbit
Grade:Purified
Purity:Purified
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Specificity:Recognizes broad range of Salmonella “O” & “H” strains. Antiserum is unadsorbed for Enterobacteriaceae. Does not crossreact with related Enterobacteriaceae.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Immunogen:Mixture of S. enteriditis, S. typhimurium and S. heildelburg