The receptors for the endothelins are found on various tissues including lung and intestine.
ETA receptor has been reported in a variety of tissues including placenta, uterus, testis, heart, adrenal gland, lung, kidney, ovary, skin, liver, bladder, large blood vessels, cerebellum, neurons, and meningioma tumor cells.
A splice variant has been identified in melanoma (Zhang et al., 1998).
ESTs have been isolated from libraries from the above-mentioned tissues.
Applications:Suitable for Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Suitable for paraffin embedded tissue
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：sheep
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Specificity：Recognizes rat Endothelin A Receptor at ~39kD. Tested using endothelin A receptor transfected COS cells for the expression of the 39kD segment of the cytoplasmic region of the receptor. Species Crossreactivity: human, mouse and sheep.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu Mo Rt Sh
Immunogen：Synthetic peptide corresponding to aa410-422, QEQNHNTERSSHK of rat Endothelin A Receptor (C-Terminal-KLH). MW: 39kD.
