MaxLight™ 750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Vascular endothelial growth factors (VEGF) are structurally and functionally related growth factors, that may play important roles in the formation of vascular systems during embryonic development, in regulation of capillary growth in adults, and in maintenance of normal vasculature.
VEGF-C is a ligand for receptor tyrosine kinase VEGFR-3 (Flt-4), which is mainly expressed in the endothelium of lymphatic vessels.
VEGF-C mRNA has been detected in human heart, lung, muscle, ovary, placenta, and small intestine.
It is also detected in many malignant tumors, including breast carcinomas, squamous cell carcinomas, lymphomas, melanomas, sarcomas and ademomas.
VEGF-C is synthesized as a precursor protein called prepropeptide, which undergoes a series of proteolytic steps to give the mature form of VEGFC.
The prepropeptide is cleaved and forms a homo-dimer between two identical propeptides.
This dimer is then processed to form a secretable tetramer that consists of two 29kD and two 31kD peptides, which is then secreted extracellularly and processed to produce the mature form that consists of two 21kD peptides.
Studies suggest the 29/31kD form is the most prevalent form of VEGF-C in many biological systems.
Applications:Suitable for use in FLISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions:FLISA (Immunogen): 0.1-1ug/mlWestern Blot (Human): 2-5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:U937 lymphoma cell lysate
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™750.
Specificity：Recognizes human VEGF-C propeptide. Does not recognize the mature form of VEGF-C.
Isotype：IgG
Calc Applications Abbrev：FLISA WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the C-terminal sequence of propeptide form of VEGF-C.
