MaxLight™ 750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Unlike endophilin 1 that is expressed in the brain and endophilin 2 that is found in the brain and testis, endophilin 3 shows a more ubiquitous expression pattern.
Endophilins have been proposed to play roles in membrane trafficking reactions through interactions with endocytic protein via physical or chemical modifications of the membrane bilayer, particularly in presynaptic vesicle trafficking at nerve terminals.
Applications:Suitable for use in FLISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions:FLISA (Immunogen): 0.1-1ug/mlWestern Blot (Mouse and rat): 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™750.
Specificity:Recognizes the mouse endophilin 3 protein. at ~40kD. Species Crossreactivity: rat. Species sequence homology: human. Reactivity has been confirmed with mouse and rat brain homogenates.
Isotype:IgG
Calc Applications Abbrev:FLISA WB
Calc Crossreactivity:Mo Rt
Immunogen:Synthetic peptide corresponding to the internal region of the mouse endophilin 3 (SH3p13) protein.