MaxLight™ 750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Connexin 40 (Cx40) is a gap junction protein highly expressed by vascular endothelial cells and in the mammalian heart, specifically in the atrial myocardium and the His-Purkinje conduction system.
At least three other connexins are expressed in the heart, including Cx37 (endocardium), Cx43 (atrial and ventricular myocardium, distal parts of the conduction system), and Cx45 (throughout the heart).
Mice lacking the gene for Cx40 exhibit reduced atrial but normal ventricular conduction velocity; these mice display right bundle-branch block and impaired left bundle-branch conduction.
Cx40 and Cx43 frequently colocalize in cells of the cardiovascular system, and participate in the formation of heterotypic gap junction channels.
For example, atrial myocytes express both Cx40 and Cx43, which oligomerize into the same hemichannel.
Atrial connexons consist of hexomeric connexin assemblies that contain varying proportions of Cx40 and Cx43 proteins.
Applications:Suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1-3ug/mlImmunofluorescence: 1-3ug/mlImmunoprecipitation: 7ug/reactionImmunohistochemistry (Frozen): 1-3ug/ml; Mice were perfused transcardially with 3ml pre-fixative (50mM phosphate buffer, 0.1% sodium nitrite, 1 unit/ml heparin), followed by 40ml fixative (0.16M sodium phosphate buffer, 0.2% picric acid, 1-, 2-, or 4% paraformaldehyde) and perfusion with 10ml sucrose wash (10% sucrose in 25mM sodium phosphate buffer, pH 7.4).
Tissues were then stored in cryoprotectant (10% sucrose, 25mM phosphate buffer, pH 7.4, 0.04% sodium azide) for a minimum of 24 hours prior to sectioning
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™750.
Specificity：Recognizes mouse Connexin 40 at ~40kD. Reactivity has been confirmed with mouse heart homogenates by Western Blot, mouse heart and lung homogenates by immunoprecipitation, and mouse heart tissue by immunohistochemistry and immunofluorescence.
Isotype：IgG
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to the C-terminal region of mouse and rat Connexin 40 (CX40) proteins. The human sequence at this location differs by only one amino acid. Species Sequence Homology: rat and human
