MaxLight™ 750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Claudin-18 is a tight junction protein expressed as lung- and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene.1 The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor.1 A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human.1 Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein.1 Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells.1 These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung andstomach.1 Claudin-18 expression has also been reported in the inner ear.2
Applications:Suitable for use in Western Blot, Immunofluorescence, and Immunohistochemistry Other applications not tested.
Recommended Dilution:Immunohistochemistry: frozen and paraffin sectionsOptimal dilutions to be determined by the researcher.
Positive Control: Mouse lung and stomach homogenates
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™750.
Specificity：Recognizes mouse Claudin-18 protein at ~29 kD. Species crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide derived from the C-terminal region of the human Claudin-18 protein, which differs from mouse by 4 conservative amino acid replacements and one non-conservative amino acid replacement
