MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Vascular endothelial growth inhibitor (VEGI) belongs to the human tumor necrosis factor (TNF) family and acts as a multifunctional cytokine.
VEGI is expressed predominantly in endothelial cells and is an endothelial cell-specific negative regulator of angiogenesis.
Human VEGI protein contains 174 amino acids.
The calculated molecular weight is approximately 20kD.
An experiment examining in vitro transcription and translation involving the use of a cDNA clone as a template revealed a similar molecular weight of approximately 22kD.
Hydrophobicity analysis predicts a 13 amino acid hydrophobic region that follows the C-terminal segment of 12 residues; this is typical of a type II transmembrane protein with the C-terminus on the extracellular surface, a single transmembrane domain, and a short cytoplasmic tail.
Northern blots reveal that VEGI transcripts have been observed in the endothelial cells of placenta, lung, kidney, skeletal muscle, pancreas, spleen, prostate, small intestine, and colon.
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilutions:FLISA: 0.1-1.0ug/ml Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™650.
Specificity：Recognizes human VEGI at~20kD. Reacts with human PC-3 prostate adenocarcinoma cell lysates.
Isotype：IgG
Calc Applications Abbrev：FLISA WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the C-terminal sequence of human VEGI.
