Sprouty-1 and -2 are membrane-anchored phosphoprotein inhibitors of growth factor signaling in endothelial cells.
Four mammalian Sprouty homologues (Sprouty-1–4) have been identified.
One study showed that overexpressed Sprouty-1 and -2 inhibits fibroblast growth factor– and vascular endothelial growth factor–induced proliferation and differentiation by repressingpathways leading to MAP kinase activation.1 Sprouty proteins are also endogenous inhibitors of the Ras/MAP kinase pathway that play an important role in the remodeling of branching tissues, such as in development of the lung, kidney tubules, vascular system, and breast ducts
A recent study shows that Sprouty-1 and –2 may have tumor suppressing activity in the breast, and found that both proteins are consistently down-regulated in breast carcinomas.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilution to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550.
Specificity：Recognizes mouse Sprouty-1 at ~34kD. A band of unknown origin was observed at ~120kD. Reactivity has been confirmed with two day-old mouse kidney homogenates. Does not recognize rat Sprouty-1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide derived from the N-terminal region of mouse and rat Sprouty-1
