特徴

• AMPK plays a key role in the regulation of energy homeostasis.
• It acts as a metabolic stress-inducing protein kinase that switches off biosynthetic pathways when cellular ATP levels are low and when 5`-AMP rises in response to fuel limitation.
• AMPK is activated by high AMP and low ATP via a mechanism that involves allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation.
• Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase.
• May increase the rate of glucose oxidation in muscle by increasing the rate of glucose transport.
• Shows homology to yeast protein kinase-encoding gene (snf1) regulating carbohydrate metabolism.
• Human AMPKg2 is a 569 aa protein, which contains unique N-terminal domains and participate in binding of AMP within the AMPK complex.
• AMPK is a heterotrimeric complex composed of a catalytic subunit and two regulatory subunits.
• Three isoforms of the gamma subunit exist in the brain with gamma 2 and gamma 3 present, which is distinct from the rat liver gamma 1 isoform.
• The noncatalytic subunits of AMPK are related to proteins that interact with snf1p; the beta subunit is related to the SIP1/SIP2/ GAL83 family of transcription regulators and the gamma subunit to SNF4.3 Mutations in AMPKgamma 2 can cause familial hypertrophic cardiomyopathy (HCM), which provides evidence for the central role of energy compromise in disease pathogenesis.
• Applications:Suitable for use in Western Blot.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™550 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.