c-MET (HGFr) is a proto-oncogene (locus 7q21-q31) protein and a cell-surface receptor of hepatocyte growth factor.
It has tyrosine kinase activity.
It consists of alpha (50kD) and beta(145kD) subunits linked by a disulfide bond.
c-Met is overexpressed in many cancers.
The MET gene is often amplified between tumorgenesis and metastasis.
It has been suggested that c-Met is useful as a prognostic marker in numerous cancers including: breast cancer, gastric carcinoma, endometrial carcinoma and others.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Frozen and formalin-fixed paraffin-embedded sections.
Epitope retrieval required using citrate buffer, pH 6.0 is required.
Enzyme digestion is not required
Optimal dilutions to be determined by the researcher.
Positive Control:Breast carcinoma
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550.
Specificity：Recognizes human c-Met. Detects a 45kD protein on Western Blots of A431 cell lysate.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the human c-Met C-terminus sequence, conjugated to a carrier protein. Expected Staining Pattern: Cytoplasmic
