Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets.
TJ strands on adjacent cells are presumed to interact with each other to form a “molecular gasket” that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other.
The 20 known claudin proteins are major structural components of tight junctions.
Applications:Suitable for use in FLISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 0.1-1.0ug/ml Western Blot: 0.5-3ug/ml Immunohistochemistry: 0.1-1.2ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550.
Specificity：Recognizes mouse Claudin-3 at 22kD.Species Crossreactivity: Mouse liver and lung tissue extract and canine MDCK and human MCF-7 cell lysates by Western Blot and formalin-fixed, paraffin-embeddedd human normal colon and colon cancer tissues by Immunohistochemistry.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Ca Hu Mo
Immunogen：A synthetic peptide from the C-terminus of mouse Claudin-3
