The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development.
Pax family members possess a DNA-binding domain encoded by the paired box.
Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program.
The paired box containing genes were first identified in Drosophila and were subsequently described in a variety of species including mouse, human, chicken, Xenopus and Zebrafish.
Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6 is associated with Aniridia, and pax-2 which is associated with Wilms tumor.
During embryogenesis, Pax-2 is expressed in the developing kidney.
In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures.
Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms’ tumors.
Persistent expression of Pax-2 in Wilms’ tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors.
Interestingly, expression of the Wilms’ tumor suppresser protein WT1 coincides with down-regulation of the pax-2 gene, and the WT1 protein can directly repress pax-2 transcription.
Therefore, pax-2 is thought to be a target gene for WT1.
Applications:Suitable for use in FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550.
Specificity：Recognizes mouse Pax2. Does not crossreact with other Pax family members. The major epitopes are located between aa204-373 of mouse Pax2. Two proteins are detected in mouse embryonic kidney lysates: a major band at 46kD (Pax2b) and a minor band at 48kD (Pax2a). Species Crossreactivity: ELISA: Immunogen; WB: Xenopus, zebrafish, embryonic mouse kidney, human Wilms’ tumors; IHC (frozen): rat, Xenopus, zebrafish, human Wilms’ tumor, mouse; IHC (paraffin): human Wilms’ tumor, mouse; IP: Embryonic mouse kidney; sheep and zebrafish.
Isotype：IgG
Calc Applications Abbrev：FLISA IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt Sh Xe Ze
Immunogen：A GST-Pax-2 fusion protein corresponding to aa188-385 from the C-terminal of mouse Pax2.
