MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Tight junctions are specialized regions of cell-cell contact that are particularly abundant in luminal epithelial cell sheets.
In freeze-fracture electron micrographs, tight junctions are visualized as belt-like bands of anastomosing sealing strands (TJ strands) that completely encircle the lateral surfaces of each cell.
TJ strands on adjacent cells interact with each other to form a "molecular gasket" that prevents ions, water and other molecules from leaking between cells and thus, from one side of the sheet to the other.
In addition to this "barrier" function, the "fence" function of tight junctions plays an important role in maintaining epithelial cell-polarity by blocking the diffusion of membrane proteins between apical (luminal) and basolateral cell surfaces.
Applications:Suitable for use in FLISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA (Immunogen): 0.1-1ug/mlWestern Blot (Canine, Human): 1-2ug/mlImmunofluorescence (Rat): 5-10ug/mlImmunohistochemistry (Human): 5-10ug/ml; For best results in IHC with formalin-fixed, paraffin-embedded tissues, heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
Specificity：Recognizes the ~22kD human Claudin-1 protein, and does not crossreact with Claudin-3. Reactivity has been confirmed with human Caco-2 and canine MDCK cell lysates and rat kidney homogenates by Western Blot and immunofluorescence, and with human skin tissue by immunohistochemistry.
Isotype：IgG
Calc Applications Abbrev：FLISA IF IHC WB
Calc Crossreactivity：Ca Hu Rt
Immunogen：Synthetic peptide corresponding to the C-terminus of the human Claudin-1 protein.
