MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Human aurora A (aurora related kinase-1 (ARK-1), Aik1, STK15, BTAK, aurora2), and human aurora B (aurora related kinase-2 (ARK-2), Aik2, STK12, aurora1) are centrosome-associated serine/threonine kinases that regulate centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis.1, 2 Aurora B has unique genetic loci relative to Aurora A suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression.1, 3 Aurora B exists in a complex with survivin and inner centromere protein (INCENP) in mitosis, and its kinase activity is stimulated by survivin binding and cell cycle-dependent phosphorylation.4 Phosphorylation of INCENP by aurora B stimulates aurora B kinase activity .5
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Immunofluorescence: 1-3ug/mWestern Blotting: 1-3ug/mlImmunoprecipitation: 7ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Epitope-affinity purified from rabbit antiserum.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
Specificity：Reacts with the human Aurora B. On Western blots, it identifies the target band at ~39kD. A band of unknown origin is also observed at ~ 75kD. Reactivity has been confirmed with human HeLa cervical carcinoma cell lysates.
Isotype：IgG
Calc Applications Abbrev：IF IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide derived from the N-terminal region of the human Aurora B protein.
