84-3658-67　Raf Kinase Inhibitor Protein (RKIP) (MaxLight 490) 100ul　R0495-51-ML490

特徴

• MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
• The Ras/Raf/MEK/ERK signaling pathway conveys mitogenic and differentiation signals from the cell membrane to the nucleus, and controls the proliferation and differentiation of different cell types.1–3 The Raf kinase inhibitor protein (RKIP) interacts with Raf1, MEK, and ERK both in vitro and in vivo,4 interfering with this signaling cascade.
• RKIP’s mechanism of action has been identified; it inhibits the Raf/MEK/ERK pathway at the Raf-1/MEK interface, where it dissociates the Raf-1/MEK complex and acts as a competitive inhibitor of MEK phosphorylation by Raf.5
• Applications:Suitable for use in FLISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:FLISA: 0.1–1.0ug/mlWestern Blot: 1–3ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™490 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.