MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
The NFkB family of induced-activated transcription factors are dimers comprised of members of the NFkB /rel protein family.
Activation of NFkB is induced by a remarkably large number of stimuli including viruses, TNF, IL-1, PMA , LPS, UV light, and others.
In most cell types, NFkB is present in the cytoplasm as a 50kD (p50) subunit and a 65kD (p65, rel A) subunit.
In unstimulated cells, nuclear localization signals present on p65 are masked by members of the IkB family of inhibitory proteins.
Subsequent to cell stimulation, IkB undergoes phosphorylation, ubiquitination and degradation by a proteosomedependant pathway, allowing nuclear translocation of the active dimeric NFkB transcription factor.
In the nucleus, NFkB binds to consensus sequences where, along with other co-factors, it participates in activating or enhancing the expression of specific genes.
Applications:Suitable for use in FLISA, Western Blot, Gel Mobility Shift Assay, Immunofluorescence/Immunocytochemistry andImmunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: paraffin sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™490.
Specificity：Recognizes human NFkB (p50). Species crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：FLISA IC IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein derived from an internal region of human NF-kB (p50).
