MaxLight™ 405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
The NFkB family of induced-activated transcription factors are dimers comprised of members of the NFkB /rel protein family.
Activation of NFkB is induced by a remarkably large number of stimuli including viruses, TNF, IL-1, PMA, LPS, UV light, and others.
In most cell types, NFkB is present in the cytoplasm as a 50 kD (p50) subunit and a 65 kD (p65, rel A) subunit.
Inunstimulated cells, nuclear localization signals present on p65 are masked by members of the IkB family of inhibitory proteins.
Subsequent to cell stimulation, IkB undergoes phosphorylation, ubiquitination and degradation by a proteosomedependant pathway, allowing nuclear translocation of the active dimeric NFkB transcription factor.
In the nucleus, NFkB binds to consensus sequences where, along with other co-factors, it participates in activating or enhancing the expression of specific genes.
Applications:Suitable for use in FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:FLISA (Immunogen): 0.1-1ug/mlWestern Blot (Human and monkey): 1-3ug/mlImmunohistochemistry (FFPE) (human): 0.5-2ug/ml.
Heat-induced epitope retrieval (Citrate buffer, pH 6.0) is requiredImmunoprecipitation: 2-5ug/IPOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™405.
Specificity：Recognizes the DNA binding subunit of human NFkB (also referred to as Rel A) at ~65kD.. Reactivity with other endogenous proteins has not been detected. Species Crossreactivity: monkey. Species sequence homology: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC IP WB
Calc Crossreactivity：Hu Mk
Immunogen：A recombinant protein derived from the carboxyl terminal portion of the human NFkB protein.
