MaxLight™ 405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Tight junctions create circumferential seals around cells in epithelial tissues that are critical for maintaining cell-cell adhesion.
The “barrier” function of tight junctions regulates the passage of ions, water, and various macromolecules through paracellular spaces, while the “fence” function maintains cell polarity.
Tight junctions thus function as a fence to prevent intermixing of molecules in the apical membrane with those in the lateral membrane.
Tight junctions are comprised of the membrane proteins occludin, claudins, and JAMs.
Claudins are responsible for the formation of tight junction strands and are connected with the actin cytoskeleton by ZO-1
Claudin-15 is expressed in endothelial cells in the mouse kidney.
Claudin-15 creates Na+ -selective paracellular channels, and charge-reversing mutations on the first extracellular domain of Claudin-15 create Cl- -selective paracellular channels.
Claudin-15 has a high level of expression in the mouse small and large intestine.
Applicaitons:Suitable for use in Immunohistochemistry, Immunofluorescence and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™405.
Specificity：Recognizes mouse Claudin-15. Reactivity has been confirmed with mouse intestine (ileum and colon) homogenates, rat ileum homogenates, and human claudin-15-transfected Xenopus oocyte homogenates. Low levels of expression have also been observed in mouse kidney homogenates by Western Blot.Species Crossreactivity: human and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to the C-terminal region of mouse Claudin-15.
