MaxLight™ 405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Ubiquitin-conjugating enzyme 9 (Ubc9)1-3 is a component of the ubiquitin-mediated proteolytic SUMO (sentrin) pathway that targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization.4,5 Ubc9 primarily mediates SUMOlation but not ubiquitinylation.6,7 An E3 ubiquitin ligase systematically transfers Ub and Ublike molecules from E2 conjugating enzymes to the targeted substrate.8 Ubc9, like other E2 family members, contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins, and the E2 member, and it shares a conserved Ubc domain of approximately 16kD.9 More than 30 different vertebrate proteins are potential candidates for SUMOlation by Ubc9.10 Many Ubc9-interacting proteins contain a PEST sequence, a stretch of at least 12 amino acids rich in P, E, D, S, and T amino acid residues.10-13 Substrates for Ubc9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, indicating that Ubc9 may regulate various cellular processes including cell cycle progression and differentiation.14,15 Many Ubc9-interacting proteins have been implicated in the maintenance of vertebrate genome integrity, including the tumor suppressor protein p53,16 the promyelocytic leukemia protein (Pml),17 the poly(ADP-ribose) polymerase PARP18 and the homologous recombination proteins Rad51/52.13,16,19 Ubc9 is predominantly present in the nucleus, and at the nuclear pore complex.10, 20-22 Ubc9’s distribution across the nuclear membrane, nuclear versus cytoplasmic, is important in controlling the subcellular localization of SUMOs and/or SUMOlated proteins, the assembly of Pml bodies, and the homologous recombination process mediated by Rad51.13
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blotting: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Epitope-affinity purified from rabbit antiserum.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™405.
Specificity：Reacts with human, mouse, rat and golden hamster ubiquitin-conjugating enzyme 9 (Ubc9) protein. On Western blots, it identifies the target band at ~18kD. A band at ~13kD is observed in mouse spleen homogenates. Reactivity has been confirmed HEK293, HepG2, HL-60 cell lysates and mouse spleen homogenates.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from an internal region of the human, mouse, rat and golden hamster Ubc9 proteins.
