84-3656-89　［取扱停止］ROC1 (Regulator of Cullins 1, Protein ZYP, RING Box 1, RING Box Protein 1, Ringbox Protein 1, Rbx, 1, RING Finger Protein 75, RNF75, ZYP Protein, Hrt1) (MaxLight 405) 100ul　R2997-80E-ML405

特徴

• MaxLight™ 405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (400nm); Emission (423nm); Extinction Coefficient 32,000.
• ROC (Regulator of Cullins) family proteins complex with cullin proteins to form ubiquitin ligases, an important step in the process of ubiquitin dependent protein degradation.
• ROC1 (also known as Rbx1 or Hrt1) is a highly conserved ring finger protein that is highly homologous to APC11, a subunit of the anaphase promoting complex.
• ROC1 is an essential subunit of CUL1 and CD53 ubiquitin ligases which catalyze ubiquitination of NFkB inhibitor, and Sic1 which is a CDK inhibitor.
• ROC1Cand ROC2 interact with all cullin proteins to form active ubiquitin ligases.
• Recent evidence suggests that ROC1 binds CUL1 and this promotes its nuclear localization.
• This eventually leads to formation of the active CUL1 ubiquitin ligase.
• Applications:Suitable for use in Western Blot, Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1–5ug/ml Immunoprecipitation: 10ug/IP reaction
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™405 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.