MaxLight™ 405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
The progressive deposition of insoluble amyloid protein plaques in specific areas of the brain and its blood vessels is associated with a number of neuropathologies and is an early invariant feature of Alzheimer’s disease.
The major component of these protein plaques is a peptide of 39-42 amino acids derived from a group of 695-770 amino acid precursor proteins collectively referred to as the b-amyloid precursor protein (Beta-APP).
b-APP is highly conserved in vertebrates; however, its normal function has not been well defined.
In each of the b-APPs, the b-Amyloid peptide (b-AP) is an internal peptide that begins about 99 residues from the carboxy-terminal end of the b-APP and extends from the extracellular domain into the middle of the hydrophobic, membrane spanning domain of the precursor protein.
The precise mechanism whereby b-AP accumulates in brain remains poorly defined.
Applications:Suitable for use in FLISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 0.1-1ug/mlWestern Blot: 1-3ug/mlImmunoprecipitation: 5-10ug; For immunoprecipitation of b-Amyloid peptide from cultured cells, it is necessary to mix the culture medium with an equal volume of 2X RIPA buffer
Immunohistochemistry: 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™405.
Specificity：Specific for the 4-5kD human b-Amyloid peptide derived by cleavage from the b-Amyloid precursor protein. Reactivity has been confirmed by Western Blot using a synthetic b-Amyloid peptide (1-40), by immunohistochemistry on Alzheimer’s diseased human brain sections and by immunoprecipitation of 35S-methionine labeled b-Amyloid peptide secreted by transfected culture cells.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC IP WB
Calc Crossreactivity：Hu
Immunogen：A 30 amino acid synthetic peptide derived from the full length (1-43 amino acid) b-Amyloid peptide.
