pGEX vectors are frequently utilized in bacterial systems to express polypeptides or entire proteins as fusions with glutathione S-transferase.
Using this fusion system, a polypeptide sequence is linked to the C-terminus of the 26 kD GST protein from Schistosoma japonicum.
The bacterially expressed GST-fusion proteins are purified by absorption with glutathione-agarose beads.
The fusion protein is subsequently eluted from the glutathione agarose with an excess of free glutathione.
Antibodies to GST are used to detect pGEX-expressed fusion proteins and as immuno-affinity reagents for purification of recombinant proteins.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA (solid phase = antigen): 0.1-1.0ug/mlELISA (solid phase = antibody): 1.0ug/mlWestern Blotting: 1ug/mlOptimal dilution determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with R-Phycoerythrin (PE).
Specificity：Specific for the ~26kD GST protein from S. japonicum and is useful for detecting GST and GST-fusion proteins transcribed from pGEX and similar vectors. Cross-reactivity with endogenous proteins from E. coli bacteria has not been observed.
Isotype：IgG
Calc Applications Abbrev：E WB
Immunogen：Purified glutathione S-transferase (GST) protein from Schistosoma japonicum expressed from a pGEX vector.
