Pax2 is a transcription factor encoded by a gene that is required for the embryonic development of a variety of mammalian tissues, including the kidney, optic cup, and inner ear.
Structurally, the Pax2/5/8 protein subfamily is characterized by an N-terminal paired domain, a conserved octapeptide sequence, and a paired-paired type homeobox.
The N-terminal paired domain of the Pax2/5/8 proteins binds DNA, while the C-terminal region is required for activation of target genes.
In human mammary tissue, Pax2 expression has been identified in sub-populations of mammary ductal cells, and also in > 50% of human breast tumors surveyed (n=38).
Mouse studies have demonstrated co-localization of Pax2 and WT1 (Wilms’ tumor suppressor protein) in the nuclei of normal and cancerous breast cells, indicating a role for Pax2 in the regulation of the response to progesterone in the mature mammary gland.
Pax2 is a phosphorylation target of JNK, and has been observed to co-imunoprecipitate with JIP1.
Phosphorylation of Pax2 enhances its ability to transactivate reporter genes.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.1-1ug/mlWestern Blot: 1-3ug/ml; Western Blot analysis of JNK-stimulated (phosphorylated) fusion proteins identifies a single band at ~50kD.
In Western Blot analysis of HEK293 cells, a thick band between ~42-45kD is observed, which may represent different human Pax2 isoforms and/or Pax8
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human Pax2 protein when phosphorylated at Ser394 in the 416aa isoform and at Ser371 of the 393 isoform. Sequence homology: Pax5 and Pax8. Does not react with purified, unphosphorylated GST-Pax2 proteins. Reacts with human HEK293 cell lysates and purified GST-Pax2 proteins phosphorylated by JNK.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phospho-peptide derived from the sequence surrounding the phosphorylated Ser393 residue of mouse and human Pax2 (paired box protein 2). Species Sequence Homology: mouse, chicken, zebrafish and frog; 100%.
