Applications:Suitable for use in ELISA, Western Blotting, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:ELISA: 0.1–1.0ug/ml Western Blotting: 1–3ug/ml Immunoprecipitation: 5–10ug
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with R-Phycoerythrin (PE).
Specificity：Specific for the 86kD CUL-1 protein and does not crossreact with the related CUL2 protein. Antibody reactivity has been confirmed by Western blotting and immunoprecipitation using both recombinanat CUL1 protein and cell lysates. Reacts with human CUL1. Reactivity with other species is possible based on sequence homology but has not been tested.
Isotype：IgG
Calc Applications Abbrev：E IP WB
Calc Crossreactivity：Hu
Immunogen：An 11 amino acid synthetic peptide derived from the extreme C-terminus of the human CUL1 protein. This peptide sequence is identical to Drosophila CUL1 (LIN-19) in 9/11 positions and in 7/11 residues with the CUL1 C. elegans and Dictyostelium discoidium homologs.
