84-3655-52　［取扱停止］STAT 5b (Signal Transducer and Activator of Transcription 5b) (PE) 100ul　S7972-15C-PE

特徴

• STATs were originally identified as two novel DNA-binding proteins (STAT1 and STAT2) which were found to play essential roles in interferon IFNa-and IFNg-regulated gene expression.2 Following the identification of these first two family members, five additional mammalian STAT proteins were cloned and characterized including: STAT3, STAT4, STAT5a, STAT5b, and STAT6 (1,2).
• All STAT proteins share several conserved structural and functional domains(1,2): (1) conserved amino terminal domain (2) DNA binding domain (3) SH3-like region (4) conserved SH2 domain responsible for: recruitment to the receptor, interaction with JAKs, STAT dimerization.
• (5) conserved tyrosine residue (Y) whose phosphorylation is required for dimerization and DNA binding (6) carboxy terminal activation domain.
• In unstimulated cells, STAT proteins exist largely in the cytoplasm as latent transcription factors.
• In response to treatment of target cells with cytokines or in some cases growth factors, specific STATs undergo tyrosine phosphorylation, homo-or heterodimerization, nuclear translocation, and DNA binding resulting in the transcriptional activation of distinct target genes(1,2).
• At least one, and oftentimes several STAT proteins are activated in response to all cytokines which utilize cytokine receptor superfamily members.
• Nevertheless, a striking specificity of STAT activation is seen in response to individual cytokines (1).
• STAT5 or MGF (mammary growth factor) was originally isolated by purification of tyrosine phosphorylated DNA binding proteins from prolactin stimulated mammary tissue and from IL-3 stimulated myeloid cells(1,2,3,4).
• Subsequently, two highly related, but distinct Stat5 genes (Stat5a and Stat5b) were identified in mouse(5,6).
• The amino acid sequences of STAT5a and STAT 5b show ~ 96% sequence similarity, and both proteins are co-expressed in most tissues of both virgin and lactating mice(5,7,10).
• However, differential accumulation of STAT5a and STAT5b mRNAs have been reported for both muscle and mammary tissue.
• STAT5 has been reported to be activated by multiple cytokines including, IL-2, IL-7, IL-9, IL-15, IL-3, IL-5, IL-6, GM-CSF, TPO, EPO, GH, and Prolactin (PRL)(1–10).
• Both the STAT5a and STAT5b proteins recognize the GAS (gamma interferon activating sequence) site TTCNNNGAA, and the preferred DNA-binding consensus site has been identified as TTCC(A>T)GGAA(1,2).
• Western blot analysis of the full-length STAT5a protein, isolated from a variety of cultured cells, indicates that the protein migrates with a molecular weight of ~95kD.
• Carboxy-terminal truncated forms of the STAT5a protein have also been reported(6,8).
• Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1–1ug/ml Immunoprecipitation: 5ug Western Blot: 1–3ug/ml Gel Mobility Shift Assay: 2.5–5ug
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.