The progressive deposition of insoluble amyloid protein plaques in specific areas of the brain and its blood vessels is associated with a number of neuropathologies and is an early invariant feature of Alzheimer’s disease.
The major component of these protein plaques is a peptide of 39-42 amino acids derived from a group of 695-770 amino acid precursor proteins collectively referred to as the b-amyloid precursor protein (Beta-APP).
b-APP is highly conserved in vertebrates; however, its normal function has not been well defined.
In each of the b-APPs, the b-Amyloid peptide (b-AP) is an internal peptide that begins about 99 residues from the carboxy-terminal end of the b-APP and extends from the extracellular domain into the middle of the hydrophobic, membrane spanning domain of the precursor protein.
The precise mechanism whereby b-AP accumulates in brain remains poorly defined.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-1ug/mlWestern Blot: 1-3ug/mlImmunoprecipitation: 5-10ug; For immunoprecipitation of b-Amyloid peptide from cultured cells, it is necessary to mix the culture medium with an equal volume of 2X RIPA buffer
Immunohistochemistry: 5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with R-Phycoerythrin (PE).
Specificity：Specific for the 4-5kD human b-Amyloid peptide derived by cleavage from the b-Amyloid precursor protein. Reactivity has been confirmed by Western Blot using a synthetic b-Amyloid peptide (1-40), by immunohistochemistry on Alzheimer’s diseased human brain sections and by immunoprecipitation of 35S-methionine labeled b-Amyloid peptide secreted by transfected culture cells.
Isotype：IgG
Calc Applications Abbrev：E IHC IP WB
Calc Crossreactivity：Hu
Immunogen：A 30 amino acid synthetic peptide derived from the full length (1-43 amino acid) b-Amyloid peptide.
