PKCs are products of multiple genes and alternative splicing that can be subdivided into several major classes, including conventional (c) PKC isoforms (alpha, beta I, beta II and gamma) and novel (n) PKC isoforms (delta, epsilon, eta and theta) and the atypical PKC (aPKC) subfamily members (sigma, iota and lambda).
Vertebrates express two atypical PKCs, sigma and lambda.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher
Positive Controls:Mouse spinal cord, adult and fetal mouse brain, and rat spinal cord homogenates.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes the ~74kD PKC lambda protein. Bands of unknown origin may be observed at ~85 and ~55kD in some homogenates.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide derived from the C-terminal region of mouse, rat and human PKC lambda.
