Pax2 is a transcription factor encoded by a gene that is required for the embryonic development of a variety of mammalian tissues, including the kidney, optic cup, and inner ear.
Structurally, the Pax2/5/8 protein subfamily is characterized by an N-terminal paired domain, a conserved octapeptide sequence, and a paired-paired type homeobox.
The N-terminal paired domain of the Pax2/5/8 proteins binds DNA, while the C-terminal region is required for activation of target genes.
In human mammary tissue, Pax2 expression has been identified in sub-populations of mammary ductal cells, and also in > 50% of human breast tumors surveyed (n=38).
Mouse studies have demonstrated co-localization of Pax2 and WT1 (Wilms’ tumor suppressor protein) in the nuclei of normal and cancerous breast cells, indicating a role for Pax2 in the regulation of the response to progesterone in the mature mammary gland.
Pax2 is a phosphorylation target of JNK, and has been observed to co-imunoprecipitate with JIP1.
Phosphorylation of Pax2 enhances its ability to transactivate reporter genes.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 0.1-1ug/mlWestern Blot: 1-3ug/ml; Western Blot analysis of JNK-stimulated (phosphorylated) fusion proteins identifies a single band at ~50kD.
In Western Blot analysis of HEK293 cells, a thick band between ~42-45kD is observed, which may represent different human Pax2 isoforms and/or Pax8
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human Pax2 protein when phosphorylated at Ser394 in the 416aa isoform and at Ser371 of the 393 isoform. Sequence homology: Pax5 and Pax8. Does not react with purified, unphosphorylated GST-Pax2 proteins. Reacts with human HEK293 cell lysates and purified GST-Pax2 proteins phosphorylated by JNK.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phospho-peptide derived from the sequence surrounding the phosphorylated Ser393 residue of mouse and human Pax2 (paired box protein 2). Species Sequence Homology: mouse, chicken, zebrafish and frog; 100%.
