84-3654-20　PTTG 1 (Pituitary Tumor-Transforming Gene 1) (HRP) 100ul　P9182-75-HRP

特徴

• Pituitary tumor-transforming gene 1 (PTTG-1) is an oncogene that is expressed in normal tissues containing proliferating cells and in tumors.
• PTTG is downstream of the MAP kinase cascade and its phosphorylation at Ser162 by MAPK in vitro.
• The phosphorylation plays an essential role in PTTG’s transactivation function.
• PTTG has a DNA-binding domain, and it activates c-Myc (an oncogene) transcription by binding to the promoter in a protein complex.
• Studies found that expression of human PTTG correlates with cell proliferation in a cell cycle-dependent manner and the association of hPTTG with Ku, the regulatory subunit of DNA-dependent protein kinase, is disrupted when a DNA double-strand breaks occurs.
• Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1-1ug/ml Western Blot: 2-4ug/ml.
• Recognizes only the 28kD band in MCF-7 cell lysate
• Immunohistochemistry: 1-2ug/ml in formalin-fixed, paraffin embedded tissues.
• For best results heat induced epitope retrieval (HIER) with citrate buffer, pH 6.0, is required prior to staining.
• Optimal dilutions to be determined by the researcher.
• Recommended Secondary Antibodies:I1904-46Q: IgG, H&L (HRP) Pab Gt xRbI1904-47J: IgG, H&L (AP) Pab GtxRtI1904-40D: IgG, H&L (FITC) Pab Gt xRbI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMoI1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.