84-3654-13　14-3-3, alpha, beta (Protein 1054, Protein Kinase C Inhibitor Protein 1, brain protein 14-3-3, beta Isoform, Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide, protein kinase C inhibitor protein-1) (HRP) 100ul　0014-02-HRP

特徴

• 14-3-3 proteins are a family of small, widely expressed, highly conserved cytosolic proteins.
• 14-3-3 proteins bind to and influence the activities of a diverse group of molecules involved in signal transduction, cell cycle regulation and apoptosis including Raf, PKC, Bad, Cbl, and c-Bcr.
• Interactions between 14-3-3 and target proteins are strongly influenced by the phosphorylation state of 14-3-3 and the target protein.
• 14-3-3 expression is particularly high in certain neural tissues and studies in Drosophila have established a role for 14-3-3 proteins in nervous system development and function.
• Recently, a dimeric 14-3-3 protein was determined to be an essential cofactor for Raf kinase activity.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilutions:ELISA: 0.1-1.0ug/mlWestern Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.