Syndecans 1-4 are heparan sulfate proteoglycans (HSPGs), which are cell-surface-associated proteins.
Syndecans contain a small core protein with distinct functional domains to which are linked multiple glycosaminoglycan (GAG) chains, predominantly of the heparan sulfate variety.
Syndecan synthesis is highly regulated and is dependent on both cell type and developmental state.
Syndecans are involved in cell adhesion, tissue morphogenesis, and differentiation and regulation of cell responsiveness to soluble growth-regulatory compounds.
These functions are attributed primarily to the glycan moieties.
Syndecans also interact with growth factors such as fibroblast growth factor and epidermal growth factor.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:ELISA (Immunogen): 0.1-1ug/ml Western Blot (Human): 1-3ug/ml; Confirmed with human T47D, SW-480 and PC-3 cell lysates.
A single band at ~90kD representing the glycosylated form of Syndecan 1 is observed and occasionally a band of ~31kD representing the unglycosylated form of Syndecan 1 may be observed.
Immunohistochemistry (FFPE): 10ug/ml; For best results heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining.
Confirmed with human gastric cancer and breast cancer tissues, as well as with mouse and rat stomach, small bowel and skin tissues.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human Syndecan 1. Species Crossreactivity (IHC): mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to an internal region of the human Syndecan 1 protein.
