SOCS-1, also called Janus kinase (JAK) binding protein (JAB), or STAT-induced STAT inhibitor (SSI-1), is an SH2-domaincontaining protein.
It was shown to interact with all four Jak kinases (Jak1, Jak2, Jak3 and Tyk2) and is induced by and inhibits the signaling action of IL-3, IL-4, IL-6, growth hormone, LIF, prolactin, interferon gamma (IFN-g), and erythropoietin.
It plays an important role in the negative regulation of IFN-g signaling and in the differentiation of T-cells.
SOCS-1 mRNA is highly expressed in the thymus, to a much lower level in spleen, and not expressed in most other tissues.
Biochemical characterization as well as gene disruption studies indicate that SOCS-1 is an important negative regulator of the JAK-STAT signal pathway.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Positive Controls:Mouse thymus cell lysates and HepG2 human hepatocellular carcinoma lysate.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes the human SOCS-1 protein at ~30kD. Species Crossreactivity: mouse Species Sequence Homology: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：A synthetic peptide derived from the C-terminal region of human, mouse or rat SOCS-1 protein
