Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), are downstream effectors of TGFb/BMP signaling.
Smad1 (MADR1) mediates signals of bone morphogenetic proteins (BMPs) in the cell.
BMPs constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.
BMP receptors are members of the TGFb family ofSer/Thr kinase receptors.
Ligand binding induces multimerization, autophosphorylation and activation of these receptors.
Subsequently, they phosphorylate Smad1 at serine 463 and 465 in the carboxyl-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites.
These phosphorylated Smads dimerize with the collaborating Smad4 (DPC4) and are translocated into the nucleus, where the transcription of target genes is stimulated.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Reacts with the human, mouse and pig Smad1 proteins. On Western blots, it identifies a single band at ~52kDa. A ~140kD band of unknown origin is seen in SK-OV-3 cell lysates and a ~200kD band of unknown origin is seen in NIH3T3 cell lysates. Reactivity has been confirmed with human HepG2 and SK-OV-3, African Green monkey COS-7, and mouse NIH3T3 cell lysates, as well as with fetal mouse muscle and thymus homogenates.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Po
Immunogen：Synthetic peptide derived from an internal region of the human Smad1 protein
