Unlike endophilin 1 that is expressed in the brain and endophilin 2 that is found in the brain and testis, endophilin 3 shows a more ubiquitous expression pattern.
Endophilins have been proposed to play roles in membrane trafficking reactions through interactions with endocytic protein via physical or chemical modifications of the membrane bilayer, particularly in presynaptic vesicle trafficking at nerve terminals.
Applications:Suitable for use in ELISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions:ELISA (Immunogen): 0.1-1ug/mlWestern Blot (Mouse and rat): 1-3ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes the mouse endophilin 3 protein. at ~40kD. Species Crossreactivity: rat. Species sequence homology: human. Reactivity has been confirmed with mouse and rat brain homogenates.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide corresponding to the internal region of the mouse endophilin 3 (SH3p13) protein.
